<i>In vitro</i> and <i>in silico</i> investigation of garlic’s (<i>Allium sativum</i>) bioactivity against 15-lipoxygenase mediated inflammopathies

Introduction: Garlic (Allium sativum) is widely used as a flavor-enhancing dietary ingredient and exhibits wide spectrum of pharmacological effects. This study aimed to investigate the therapeutic effects aqueous garlic extract explore bioactivity against 15-lipoxygenase (15-LOX) mediated inflammopathies. Methods: In this study, antioxidant (DPPH free radical scavenging assay reducing power assay), anti-inflammatory (hypotonicity-induced hemolysis 15-LOX inhibition assay) anticoagulation (serine protease prothrombin time were investigated. Furthermore, in silico molecular docking dynamic simulation analysis reported small compounds 15-LOX1 15-LOX2 performed figure out most efficient phytochemical ligands validate potential. Results: The DPPH effect found with IC50 213.87 ± 1.49 μgmL-1 EC50 124.78 3.39 μgmL-1, respectively. hypotonicity-induced assay, values observed 147.59 2.98 250.05 8.48 inhibited serine activity an 301.33 1.31 prevented blood coagulation for 10.05 0.35 minutes assay. identified Rhamnetin potential inhibitor, it exhibited stable interaction targets throughout 100 ns simulation. Conclusion: findings provide insights into garlic’s medicinal properties well its bioactive compounds, which can be interventions inflammations.